Advancing excellence in laboratory medicine for better healthcare worldwide

Vol 18 n° 4


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Assessment of Plasma S-Nitrosothiol Concentration by Electron Paramagnetic Resonance Spectrometry and Plasma Nitrotyrosine Levels by ELISA in Beh�et's Disease


Introduction

Behçet's Disease (BD) is a chronic inflammatory, multisystemic disease characterized by oral, genital ulcerations, uveitis, vasculitis, and arthritis. BD is endemically higher in the Republic of Turkey, the Middle East, and Japan compared with other countries (1, 2, 3).
Although the exact underlying mechanism of BD is still unknown, recent findings have focused on the role of reactive oxygen species (ROS) produced by activated neutrophils and other inflammatory cells during inflammation (4,5). Recently, it has been also shown that nitrite and nitrate concentrations, used as indirect markers of nitric oxide (NO) production are increased in BD patients and associated with disease activity (6,7,8). NO produced by endothelial cells may be an important mediator in the development of BD.
However, human blood nitrite/nitrate measurements may be confounded by dietary intake of nitrate and nitrite (9). Some studies have also shown that S-nitrosothiols, which are bioactive forms of NO, are increased in certain inflammatory diseases such as RA (rheumatoid arthritis), and are associated with the disease activity (11). S-nitrosothiols are derived from NO by S-nitrosation of thiol-containing proteins. S-nitrosothiol formation may prevent the formation of peroxynitrite that results from the reaction between NO and superoxide anion (O2-) through sequestration of available NO (10,11). Although S-nitrosothiols have been measured by various techniques, electron paramagnetic resonance spectrometry, in conjunction with spin trapping, is considered to be a more sensitive and specific method (11). Recently, nitrotyrosine (3-NT) has been identified as another marker of NO production. Nitrotyrosine is a relatively stable product and forms from the reaction of peroxynitrite with free and protein bound tyrosine. It has been found to be elevated in various inflammatory processes such as atherosclerosis, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock (12)

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