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PSEP Report Aysegul Hanikoglu - TR

THE IFCC PROFESSIONAL SCIENTIFIC EXCHANGE PROGRAMME (PSEP), MODENA, ITALY EXPERIENCE

by Aysegul Hanikoglu, Akdeniz University Faculty of Medicine,
Department of Clinical Biochemistry Antalya-Turkey

I recently had the great experience of being the awardee of the IFCC - PROFESSIONAL SCIENTIFIC EXCHANGE PROGRAMME (PSEP) scholarship. I had the chance to spend two months (from 13 June 2016 to 12 August, 2016) in Clinical Pathology and Laboratory Medicine, Università di Modena e Reggio Emilia, Modena, supported by the IFCC PSEP program

The project was focussed on the analysis of plasma circulating DNA at diagnosis and during follow-up of prostate cancer. The study’s aim was to establish the correlation between PSA and cfDNA. We hypothesized a significant effect of this correlation on disease outcome will indicate that a composite molecular assessment in blood may be a useful determinant of disease status and efficacy of systemic therapy for prostate cancer. Lastly, we can detect and evaluate preoperative and postoperative cfDNA concentration and hypermethylation of tumor suppressor genes by qPCR quantification method of prostate cancer patients. The IFCC - PROFESSIONAL SCIENTIFIC EXCHANGE PROGRAMME (PSEP) provided me to learn how to perform;

  • Detection of cancer-specific DNA mutation signatures and/or epigenetic modifications
  • Genotype analysis focused on the detection of cancer-specific DNA mutations in prostate cancer patients
  • cfDNA extraction
  • Quantification of cfDNA using two different methods ( Qubit 2.0 and Nanodrop fluorometer  measurements).

We performed cfDNA extraction with QIAamp® Circulating Nucleic Acid kit. I learned these techiques very well. So, I can collaborate on a common research project where I was trained to apply qPCR quantification method and techniques to identify novel biomarkers in prostate cancer patients before and after (at 6 and 12 months) the operation. During my visit, I also learned some Advanced Diagnostic Techniques used in the host laboratory such as mass spectrometry, proteomics and genomics techniques applied to Clinical Laboratory Medicine.

I would like to thank firstly Prof. Dr. Aldo Tomasi for giving me the opportunity to work on this project in Modena. I also want to thank to Dr. Giovanni Ponti and Monia Maccaferri. I was very lucky to be a part of this team. I learned a lot and I will always be grateful for this opportunity, work environment and new friendships I gained throughout this program. I would also like to thank to Emanuela Monari, Elisa Bellei, Stefania Bergamini, they were very kind and helpful. I am very grateful and thankful again since they shared their time, work experiences with me and helped me during my stay in Modena. I wish to thank the IFCC for giving me the opportunity to participate as a scholar of this PSEP program.

 PSPE AysegulhanikogluAysegul Hanikoglu in Modena

 
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